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The 49 U.S. AI Startups Securing $100M+ in Funding This Year

AI Funding in 2025: A New Wave of Mega-Rounds Reshapes the Industry

A Record-Breaking Momentum in AI Investment

Last year set an extraordinary benchmark for artificial intelligence in the United States and around the world. According to TechCrunch, “There were 49 startups that raised funding rounds worth $100 million or more in 2024… three companies raised more than one ‘mega-round,’ and seven companies raised rounds that were $1 billion in size or larger.”

As 2025 nears its end, the pace hasn’t slowed. In fact, the year has already matched 2024’s total number of $100 million-plus rounds, while also recording more companies securing multiple mega-rounds than the year prior.

Below is a month-by-month breakdown of every U.S.-based AI startup that has raised $100 million or more in 2025.


Major AI Funding Rounds of 2025


📅 November

  • Anysphere – Raised $2.3 billion, valuing the company at $29.3 billion. Announced November 13; the company’s second round this year.

  • Parallel – Closed a $100 million Series A on November 12, co-led by Index Ventures and Kleiner Perkins.

  • Hippocratic AI – Secured $126 million Series C, valuing the company at $3.5 billion. Announced November 3; its second round of 2025.


📅 October

  • Fireworks AI – Raised $250 million Series C on October 28 at a $4 billion valuation.

  • Uniphore – Announced $260 million Series F on October 22; valuation: $2.5 billion.

  • Sesame – Closed a $250 million Series B on October 21, co-led by Sequoia and Spark Capital.

  • OpenEvidence – Raised $200 million Series C on October 20, valued at $6 billion.

  • Lila Sciences – Announced a $350 million Series A on October 14.

  • Reflection AI – Closed a $2 billion Series B on October 9, valuing the startup at $8 billion.

  • EvenUp – Raised a $150 million Series E on October 7 at a valuation above $2 billion.


📅 September

  • Periodic Labs – Closed a $300 million seed round on September 30.

  • Cerebras Systems – Raised a $1.1 billion Series G at an $8.1 billion valuation.

  • Modular – Announced a $250 million round on September 24.

  • Distyl AI – Secured a $175 million Series B on September 23 at a $1.8 billion valuation.

  • Upscale AI – Raised a $100 million seed round on September 17.

  • Groq – Closed a $750 million Series E, valuing it at nearly $6.9 billion.

  • Invisible Technologies – Raised $100 million on September 16 at a $2 billion valuation.

  • Cognition AI – Secured a $400 million Series C on September 8; valuation: $10.2 billion.

  • Baseten – Closed a $150 million Series D on September 5, valuing the company at $2.1 billion.

  • Sierra – Raised $350 million on September 4; valuation: $10+ billion.

  • You.com – Closed a $100 million Series C on September 3 at $1.5 billion.

  • Anthropic – Raised a $13 billion Series F on September 2, valuing the lab at $183 billion.


📅 August

  • EliseAI – Closed a $250 million Series E on August 20 at a $2.2 billion valuation.

  • Decart – Raised $100 million, pushing valuation to $3.1 billion.


📅 July

  • Fal – Raised $125 million Series C on July 31 at a $1.5 billion valuation.

  • Ambience Healthcare – Announced a $243 million Series C.

  • Reka AI – Raised $110 million Series B on July 22, valuing the company at $1 billion.

  • Thinking Machines Lab – Confirmed a $2 billion seed round on July 15; valuation: $12 billion.

  • OpenEvidence – Raised $210 million Series B on July 15.

  • Harmonic – Closed a $100 million Series B on July 10.


📅 June

  • Abridge – Secured a $300 million Series E at a $5.3 billion valuation.

  • Harvey – Raised its second $300 million round of 2025 on June 23.

  • Tennr – Closed $101 million Series C led by IVP.

  • Glean – Raised $150 million Series F on June 10 at a $7.25 billion valuation.

  • Anysphere – Raised $900 million Series C, pushing valuation near $10 billion.


📅 May

  • Snorkel AI – Closed a $100 million Series D on May 29.

  • LMArena – Raised $100 million seed round on May 21 at a $600 million valuation.

  • TensorWave – Raised $100 million Series A on May 14.


📅 April

  • SandboxAQ – Closed a $450 million Series E on April 4; valuation: $5.7 billion.

  • Runway – Raised $308 million Series D on April 3 at a $3 billion valuation.


📅 March

  • OpenAI – Raised a record $40 billion round, valuing the company at $300 billion.

  • Nexthop AI – Raised $110 million Series A on March 25.

  • Insilico Medicine – Closed a $110 million Series E.

  • Celestial AI – Raised $250 million Series C at a $2.5 billion valuation.

  • Lila Sciences – Raised $200 million seed round.

  • Reflection.Ai – Closed $130 million Series A, valuing it at $580 million.

  • Turing – Raised $111 million Series E on March 7.

  • Shield AI – Closed $240 million Series F on March 6, valuing the startup at $5.3 billion.

  • Anthropic – Raised $3.5 billion Series E on March 3, valuing the company at $61.5 billion.


📅 February

  • Together AI – Raised $305 million Series B at a $3.3 billion valuation.

  • Lambda – Closed a $480 million Series D on February 19.

  • Abridge – Raised $250 million Series D on February 17 at $2.75 billion.

  • Eudia – Closed a $105 million Series A.

  • EnCharge AI – Raised $100 million Series B on February 13.

  • Harvey – Closed $300 million Series D on February 12 at $3 billion.


📅 January

  • ElevenLabs – Secured $180 million Series C, valuing the company at *$3 billion+.

  • Hippocratic AI – Announced a $141 million Series B on January 9, valuing it at $1.6 billion+.


Conclusion: Another Transformative Year for AI

From billion-dollar seed rounds to unprecedented mega-fundraises across healthcare, infrastructure, scientific research, and enterprise automation, 2025 demonstrates that investor appetite for AI innovation remains extraordinarily strong.

As updates continue to roll in throughout the year, the trajectory suggests that 2025 could ultimately surpass even 2024’s “monumental” figures.

Din Kumar
Author: Din Kumar

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